Dear Mr. Tvedten:

On August 16, 2008 we received from you fabric to be micro tested.  The two pieces of fabric were labeled as “original” and “treated”.

With aseptic technique we cut approximately 1 gram from each sample and added these to a sterile tube containing TAT Broth Base for cultivation of microorganisms from highly viscous or gelatinous materials.

From the above tube we extracted, with a sterile pipette, 2 mls and placed 1 ml into each of two sterile petri dishes.   In one of the petri dishes we added (TSA) Tryptic Soy Agar (Soybean-Casein Digest Agar) Base for a general purpose medium.  In the second plate we added (SA) Sabouraud Dextrose Agar Base for the cultivation of fungi and aciduric microorganisms.

Plates tested with Tryptic Soy Agar were incubated for 72 hours at 37°C (+/- 2°C).  Plates tested with Sabouraud Dextrose Agar were incubated for 5 days at 25°C (+/- 2°C).

Tests were conducted two times under the same procedures (Results 1 & 2, 1A & 2A respectively).  The results and conclusions are attached for your review.

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Test Results

Test #          Description        TSA          Count            SA              Count

    1              Original             Positive    140cfu/gm     Positive      TNTC

    2              Treated              Positive      30cfu/gm     Negative     <10cfu/gm

    1A           Original             Positive    110cfu/gm     Positive      TNTC

    2A           Treated              Positive      10cfu/gm     Negative     <10cfu/gm

Description

Positive = Micro contamination present

Negative = No micro contamination is present

TNTC = Too Numerous To Count

Conclusions

After the tests were performed, our conclusions indicate that samples described as “treated” were less susceptible to bacteria/fungi growth.

Samples labeled “original” had bacteria present.

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Bacteria and mold were retarded even though the twice patented biopesticide (Safe Solutions Enzyme Cleaner with Peppermint) was dry and simply laid on the petri dishes - the control of bacteria and mold results would have been even better if the enzyme cleaner was  used as a spray or as a cleaner.

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