Part 2.

Results
The examination of the medical indexes shows that during the bigu-fasting
trial, the subjects were in a state of negative metabolic balance. For the body
tolerance to a long term negative nitrogen balance, the scientific data obtained
from the two subjects has a very important value.

Table 2.
Metabolic consumption and nitrogen balance
_____________________________________________________
Observed items Calorie metabolism (kcal/day) Nitrogen balance (g/day)

consumption balance consumption balance
_____________________________________________________

Xiao Ding 1511.5 - 1205.4 2.2 - 2.0
_____________________________________________________

Wang Jianjun 1800.1 - 1315.0 4.3 - 4.2
_____________________________________________________

The scientific data also clearly indicates that although the two subjects
have lived 30 days with a substantially low nutrient intake and a serious
negative nitrogen balance, still there are no indicators that within their
bodies have occurred organic impairment. The internal environment of the
subjects has also maintained a relative homeostasis and normal blood flow
indexes. The biochemicall data shows that blood pH, blood sugar, blood lipids,
blood serum, the immunology points and the hematological index of the subjects
were within the normal scope. Hemalbumin and metabolites were all normal, with
a slight increase of creatine.


Table 3.
Daily excretory amount
___________________________________________________
Observed items Xiao Ding Wang Jianjun Normal index
___________________________________________________
Amino acids (mg)

isoleucine 1.8 2.9 10 - 30

leucine 2.3 2.6 10 - 25

lysine 4.3 3.6 10 - 50

phenylalanine 4.4 57.0 10 - 30

glycine 46.8 44.7 70 - 200

alanine 11.0 6.5 20 - 70

tyrosine 3.9 5.3 15 - 50

histidine 29.4 23.3 110 - 320

Mineral common elements (mg)

K 295.8 525.4 2000 - 4000

Na 253.7 264.6 3000 - 5000

Ca 26.4 47.5 200 - 300

Mg 29.9 56.9 80 - 120

P 206.6 500 - 1300

Mineral trace elements (mg)

Zn 659.6 1683.1 400 - 500

Cu 24.9 28.1 40 - 70

Fe 30.5 63.8 800 - 1000

Mn 0.6 2.9 10 - 20

Cr 1.1 2.9 3.6 - 22
___________________________________________________

The biochemical study of the excretory amount of the observed subjects shows
a substantial decrease of urinary and fecal discharge of nutrients.

Table 4.
Energy metabolism and respiratory quotient in the state of sitting relaxation
___________________________________________________
Observed items Metabolism rate (kcal/min) respiratory quotient (RQ)
___________________________________________________
Xiao Ding 0.14 - 0.21 0.44 - 0.50

Control group 0.46 - 0.56 0.67 - 0.60
___________________________________________________
Wang Jianjun 0.38 0.51 - 0.57

Control group 1.21 - 1.26 0.80 - 0.92
___________________________________________________

The clinical study of the energy metabolism and respiratory quotient rate of
Xiao Ding and Wang Jianjun are notably lower than the control group of their sex
and age. The basal metabolism rate (BMR) of Wang Jianjun falls from the normal
1.21 kcal to 0.38 kcal, and in Xiao Ding - from 0.48 kcal to 0.14- - 0.21. It
is understandable that by decreasing the metabolism rate, the body could save
energy. Similarly, in the state of sitting relaxation the respiratory quotient
of the subjects decreases approximately by 40%. The process of RQ decrease is
carried out along with a mobilization of triacy glycerols from the fat depots,
which can stimulate the transformation of body fat into glycogens - an energy
that is more convenient for use by the body.

Table 5.
Salivation amount and amylase activity after two weeks of bigu -fasting
_____________________________________________________
Observed items Mastication method Salivation amount Amylase activity
(ml/min) (KU/L)
_____________________________________________________________________
Xiao Ding Simple mastication 0.11 451.4

Acid stimulation 0.30 895.7
_____________________________________________________________________
Wang Jianjun Simple mastication 0.04 -0.06 61.0-88.0

Acid stimulation 0.12 -0.22 80.0-105.0
_____________________________________________________________________
Control Group Simple mastication 0.60 - 0.90 620.0-682.9

Acid stimulation 0.60 737.5-882.2
____________________________________________________________________

The electrogastric activity (EGA) amplitude of Xiao Ding and Wang Jianjun in
the state of laying relaxation are notably lower than the indexes of the control
group. Several seconds after intaking a definite amount of liquids, the
electrogastric activity amplitude of the control group highly increased, while
Wang Jianjun and Xiao Ding’s reaction to this kind of stress was relatively
weak. The constrainment of the electrogastric activity amplitude clearly shows
the change of the disusability of the stomach, created by the bigu-fasting.
During the clinical observation period, Xiao Ding repeatedly developed
strong positive ketonuria with a very high numerical value of the ketone bodies.
Wang Jianjun also showed high ketonuria in the first three days of the
experiment. When there was a very low amount of calorie and liquid intake, the
ketonuria appeared; as soon as there was an increased supply of calorie and
liquid intake, the ketonuria disappeared immediately. Taking into account that
Xiao Ding and Wang Jianjun were, on the whole, in a good state and stable
homeostasis, this type of ketonuria caused by low nutrient intake not
necessarily represents a morbid condition, but is actually a natural outcome of
a major mobilization of the bodily fat. In the absence of dietary sugar, there
is a great utilization of fat for energy. The ketonuria effect of prolonged
bigu-fasting is encountered in such diseases as diabetes mellitus where glucose
cannot be properly utilized for energy purposes. Yet it is different from the
ketosis as seen in diabetes mellitus.

During the 30-day clinical observation period of the trial, Xiao Ding lost
4,4 kg and Wang Jianjun -- 5.5 kg. The weight decrease actual measured value of
the subjects is much lower than the normal energy and substance metabolism
calculated formula of the bodily weight decrease expected value. Only after
deducting the portion made up by the dehydration, could be determined the
actual value of the bodily depletion. That is why, the bodily depletion of the
observed group in fact is not as serious as it seems. This is also helpful to
understand the stability of their homeostasis and state of physical and mental
well-being. The observed subjects were able to follow their usual daily
activities without any presence of discomfort or fatigue.

After completing the clinical study, both Xiao Ding and Wang Jianjun have
actively participated in their everyday life activities, without a record of
getting ill. Their body weight is stable and they enjoy a normal physiological
and spiritual life.

Discussion
From the point of view of the physiological health, the bigu-fasting is
applied as a natural method of therapy, but not in cases of children and adults
with weak constitution. The former, as it is known, are in a period of intense
growth, when every shortage of nutrients may lead to consequences in one or
another ways; the latter - the people with qi insufficiency and weakened by
prolonged diseases organism, as a result of the fasting their energy may deplete
to very low levels, even if there might occur some improvements in the
physiological state. That is why, in cases with children and adults with weak
constitution, the Chinese medicine proposes other natural ways of treatment -
for example through the choice of food intake, which also has a healing effect,
through herbal therapy, massage, qigong therapy and others.

There exist many specific ways and levels of this method. It is very
important that the extent and duration of fasting should be determined by
specialized physicians. It is never applied drastically.

It should be clear that through bigu-fasting the energy cannot be developed,
but only are created conditions for its better circulation and work.


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