NeuroRepair Department, Medical Research Institute, Polish Academy of Sciences,
Warsaw, Poland. buzanska@...
The expanding population of neural stem/progenitor cells can be selected from
human cord blood nonhematopoietic (CD34-negative) mononuclear fraction. Due to
repeated expansion and selection of these cells we have established the first
clonogenic, nonimmortalized human umbilical cord blood neural stem-like cell
(HUCB-NSC) line. This line can be maintained at different stages of neural
progenitor development by the presence of trophic factors, mitogens and
neuromorphogens in culture media. Neurogenic potential of HUCB-NSC was
established for serum-free and low-serum cultured cells. Commitment of HUCB-NSC
by serum was shown to be important for the optimal response to the signals
provided by surrounding environment in vitro. Enhanced neuronal differentiation
induced by dBcAMP treatment was accompanied by expression of several functional
proteins including glutamatergic, GABAergic, dopamine, serotonin and
acetylcholine receptors, which was shown by microarray, immunocytochemistry and
electrophysiology. Electrophysiological studies, whole-cell patch-clamp
recordings, revealed in differentiated HUCB-NSC two types of voltage-sensitive
and several ligand-gated currents typical for neuronal cells. The above
HUCB-NSC characteristic conceivably implicates that cord blood-derived
progenitors could be effectively differentiated into functional neuron-like
cells in vitro. Copyright (c) 2006 S. Karger AG, Basel.
Harvard scientists identify compounds that stimulate stem cell growth in the brain BETHESDA, Md.--Scientists at Harvard University have identified key ...
LONDON, England (UPI) -- In what mark a revolutionary breakthrough for both laboratory research and hospital treatment, a team of British scientists has grown...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
Offline 
Send Email