Acta Biol Hung. 2005;56(3-4):233-45. Related Articles, Links

Detection of nanobacteria-like particles in human atherosclerotic plaques.

Puskas LG, Tiszlavicz L, Razga Z, Torday LL, Krenacs T, Papp JG.

Laboratory of Functional Genomics, Biological Research Center, Hungarian
Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary.
pusi@...

Recent and historical evidence is consistent with the view that
atherosclerosis is an infectious disease or microbial toxicosis impacted by
genetics and behavior. Because small bacterial-like particles, also known as
nanobacteria have been detected in kidney stones, kidney and liver cyst
fluids, and can form a calcium apatite coat we posited that this agent is
present in calcified human atherosclerotic plaques. Carotid and aortic
atherosclerotic plaques and blood samples collected at autopsy were examined
for nanobacteria-like structures by light microscopy (hematoxylin-eosin and a
calcium-specific von Kossa staining), immuno-gold labeling for transmission
electron microscopy (TEM) for specific nanobacterial antigens, and
propagation from homogenized, filtered specimens in culture medium.
Nanobacterial antigens were identified in situ by immuno-TEM in 9 of 14
plaque specimens, but none of the normal carotid or aortic tissue (5
specimens). Nanobacteria-like particles were propagated from 26 of 42
sclerotic aorta and carotid samples and were confirmed by dot immunoblot,
light microscopy and TEM. [3H]L-aspartic acid was incorporated into high
molecular weight compounds of demineralized particles. PCR amplification of
16S rDNA sequences from the particles was unsuccessful by traditional
protocols. Identification of nanobacteria-like particles at the lesion
supports, but does not by itself prove the hypothesis that these agents
contribute to the pathogenesis of atherosclerosis, especially vascular
calcifications.

PMID: 16196199 [PubMed - indexed for MEDLINE]

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstra\
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