250 mg/kg bw aspartame for 28 days affects serum creatinine and harms kidneys in
male mice, Umi Barokah Irawati, abstract: Murray 2007.05.03
http://groups.yahoo.com/group/aspartameNM/message/1418

http://260684.blogspot.com/2007/05/effect-aspartame.html

My research

Thursday, May 3, 2007

Effect aspartame

THE EFFECT OF ASPARTAME TO THE SERUM CREATININE LEVEL AND HISTOLOGIC STRUCTURE
OF KIDNEY IN MICE (Mus musculus L.) STRAIN SWISS

Umi Barokah Irawati

ABSTRACT

The aims of this research were to know the effect of aspartame to the serum
creatinine level and histologic structure of kidney (glomerules and proximal
tubules).

This research was evaluated experimentally to male mice with average weight of
20 gr.

These mice were divided randomly in to 5 groups i.e.: control, 5 mg aspartame,
10 mg aspartame, 15 mg aspartame, and 20 mg aspartame.

The treatment was done for 28 days, before treatment blood had taken and
measured creatinine serum level.

After treatment mice blood had taken again, killed and sectioned kidney organ to
make histologic preparation.

Creatinine serum level, wide average Bowman space glomeruli and lumen proximal
tubules was analyzed
using analysis of varians (ANOVA) and continued Duncan Multiple Range Test
(DMRT) on 5% significans,
time factor of creatinine serum level was analyzed using T-test.

Observation of kidney histologic structure (glomerules and proximal tubules)
were done to determine the kind and level of damage and analyzed descriptively.

Creatinine serum level control normaly,
dosage variation aspartame creatinine serum level increased up to ± 3.29 mg/dl.

There were control glomerules and proximal tubules normaly.

Dosage variation aspartame glomerules damage, i.e.:
cloudy swelling degeneration; karyorectic; picnotic; karyolitic; wide Bowman
space and hemolitic erytrocyte,
proximal tubules damages were picnotic; karyorectic; karyolitic; hydrophic
degeneration; lumen narrowed and protein cast.

Key Word : aspartame, creatinine, glomerules, proximal tubules

Posted by biologi at 2:21 AM

1 comments:

Rich Murray said...

This is extremely valuable research.

Please put the entire text on your blog, along with microphotographs of the
tissues.

You may choose to ask for help in the translation into English.

It appears that you find toxic effects to the kidney at the lowest dose of
250 mg aspartame per kg body weight.
Mice and rats are about ten times more resistant to methanol (formaldehyde)
toxicity than humans.

How many mice did you test?

You may choose to use Dimethyl Dicarbonate as a palatable source of methanol
to do the same study to find if methanol is the part of aspartame that damages
kidney tissues in mice:

http://groups.yahoo.com/group/aspartameNM/message/1052
DMDC: Dimethyl dicarbonate 200mg/L in drinks adds methanol
98 mg/L ( becomes formaldehyde in body ): EU Scientific
Committee on Foods 2001.07.12: Murray 2004.01.22


formaldehyde as a potent unexamined cofactor in cancer research -- sources
include methanol, dark wines and liquors, aspartame, wood and tobacco smoke:
IARC Monographs on the Evaluation of Carcinogenic Risks to Humans implicate
formaldehyde in #88 and alcohol drinks in #96: some related abstracts: Murray
2007.04.30
http://groups.yahoo.com/group/aspartameNM/message/1417

[ See also:

http://groups.yahoo.com/group/aspartameNM/message/1286
methanol products (formaldehyde and formic acid) are main
cause of alcohol hangover symptoms [same as from similar
amounts of methanol, the 11% part of aspartame]:
YS Woo et al, 2005 Dec: Murray 2006.01.20


http://groups.yahoo.com/group/aspartameNM/message/1143
methanol (formaldehyde, formic acid) disposition:
Bouchard M et al, full plain text, 2001: substantial
sources are degradation of fruit pectins, liquors,
aspartame, smoke: Murray 2005.04.02 ]


" Absorbed formaldehyde can be oxidized to formate and carbon dioxide or can
be incorporated into biologic macromolecules. "

[ References include: Soffritti M, Belpoggi F, Lambertini L, Lauriola M,
Padovani M, Maltoni C. 2002. Results of long-term experimental studies on the
carcinogenicity of formaldehyde and acetaldehyde in rats. Ann NY Acad Sci
982:87-105.

Soffritti M, Maltoni C, Maffei F, Biagi R. 1989. Formaldehyde: an
experimental multipotential carcinogen. Toxicol Ind Health 5:699-730. "

Morando Soffritti is a member of the Working Group. ]


aspartame (methanol, formaldehyde) toxicity research summary: Rich Murray
2007.05.03
http://groups.yahoo.com/group/aspartameNM/message/1404

One liter aspartame diet soda, about 3 12-oz cans,
gives 61.5 mg methanol,
so if 30% is turned into formaldehyde, the formaldehyde
dose of 18.5 mg is 37 times the recent EPA limit of
0.5 mg per liter daily drinking water for a 10-kg child:
www.epa.gov/teach/chem_summ/Formaldehyde_summary.pdf
2007.01.05 [ does not discuss formaldehyde from methanol
or aspartame ]
http://www.epa.gov/teach/teachsurvey.html comments
teach@...


"Of course, everyone chooses, as a natural priority,
to actively find, quickly share, and positively act upon
the facts about healthy and safe food, drink, and
environment."

Rich Murray, MA Room For All rmforall@...
505-501-2298 1943 Otowi Road, Santa Fe, New Mexico 87505

http://groups.yahoo.com/group/aspartameNM/messages
group with 74 members, 1,418 posts in a public, searchable archive
http://RMForAll.blogspot.com

http://groups.yahoo.com/group/aspartameNM/message/1340
aspartame groups and books: updated research review of
2004.07.16: Murray 2006.05.11


http://groups.yahoo.com/group/aspartameNM/message/1395
Aspartame Controversy, in Wikipedia democratic
encyclopedia, 72 references (including AspartameNM # 864
and 1173 by Murray), brief fair summary of much more
research: Murray 2007.01.01


Dark wines and liquors, as well as aspartame, provide
similar levels of methanol, above 120 mg daily, for
long-term heavy users, 2 L daily, about 6 cans.

Within hours, methanol is inevitably largely turned into
formaldehyde, and thence largely into formic acid -- the
major causes of the dreaded symptoms of "next morning"
hangover.

Fully 11% of aspartame is methanol -- 1,120 mg aspartame
in 2 L diet soda, almost six 12-oz cans, gives 123 mg
methanol (wood alcohol). If 30% of the methanol is turned
into formaldehyde, the amount of formaldehyde, 37 mg,
is 18.5 times the USA EPA limit for daily formaldehyde in
drinking water, 2.0 mg in 2 L average daily drinking water.
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