Ann Plast Surg 2001 Nov;47(5):540-6 Related Articles, Books, LinkOut


Protective effect of fucoidin (a neutrophil rolling inhibitor) on ischemia
reperfusion injury: experimental study in rat epigastric island flaps.

Cetin C, Kose AA, Aral E, Colak O, Ercel C, Karabagli Y, Alatas O, Eker A.

Department of Plastic & Reconstructive Surgery, Osmangazi University Medical
Faculty, Eskisehir, Turkey.

The objective of this study was to examine whether a decrease in
neutrophil-mediated tissue injury using Fucoidin, a nontoxic neutrophil rolling
inhibitor, would improve flap survival in an island flap model after
ischemia-reperfusion. Myeloperoxidase activity (an indirect index of tissue
neutrophil count) and malondialdehyde (an indicator of lipid peroxidation), the
degree of neutrophil infiltration by direct counting, and macroscopic flap
survival were assessed in the flap after arterial ischemia-reperfusion.
Epigastric island skin flaps were elevated in 56 rats. The first group of 21
rats was subjected to 6 hours of arterial ischemia. The second group of 21 rats
was subjected to 10 hours of arterial ischemia, and the rest of the rats were
used as nonischemic controls (sham flaps). For inhibiting neutrophil rolling, a
nontoxic polysaccharide agent-Fucoidin-was used. Each ischemic group was divided
further into three subgroups: Subgroup I (control rats) received saline,
subgroup II received 10 mg per kilogram Fucoidin, and subgroup III received 25
mg per kilogram Fucoidin before reperfusion. The results were evaluated as
tissue neutrophil counts, tissue malondialdehyde content, tissue myeloperoxidase
activity, and flap survival. Neutrophil counts and tissue myeloperoxidase
activity were decreased significantly (p <0.001) in subgroup III, but lipid
peroxidation by means of tissue malondialdehyde content was not affected by
Fucoidin administration. The authors conclude that administration of Fucoidin
before reperfusion can limit tissue injury apparently by inhibiting neutrophil
rolling in a dose-dependent manner.

PMID: 11716267 [PubMed - indexed for MEDLINE]