[Lipoprotein lipase and serum thymidine kinase level in chronic lymphocytic
leukemia and their correlations with other prognostic factors]
W Xu, QD Shen, H Yu, C Qiao, YJ Wu, Q Liu, DX Zhu, KR Miao, and JY Li
Zhonghua Xue Ye Xue Za Zhi, January 1, 2009; 30(1): 8-12.

Department of Hematology, First Affiliated Hospital of Nanjing Medical
University, Jiangsu Province Hospital, Nanjing 210029, China.

OBJECTIVE: To investigate lipoprotein lipase (LPL) and serum thymidine kinase
(TK) levels in chronic lymphocytic leukemia (CLL) and their correlations with
other prognostic factors. METHODS: LPL expression level in peripheral blood
samples of 58 CLL patients was detected by semi-quantitative reverse
transcription PCR (RT-PCR). Serum TK1 level in 39 CLL patients was detected by
enhanced chemiluminescence (ECL) and TK monoclonal antibody (Anti-TK mAb). IgVH
mutation status was detected by multiplex PCR and sequencing of purified PCR
products. The expression of ZAP-70 protein and CD38 were determined by flow
cytometry . Panel probes and fluorescence in situ hybridization (FISH) were used
to detect cytogenetic aberrations. RESULTS: The median LPL expression level in
CLL was 0.26 (0 -6.29), while undetectable in normal controls. LPL expression
level was significantly correlated with IgVH mutation status, Binet stages, CD38
and cytogenetic aberrations. Patients with unmutated IgVH genes had higher LPL
than those with IgVH mutations (P = 0.010). Patients in Binet stage B and C had
higher LPL than those in stage A (P = 0.011). LPL level was higher in patients
with CD38 > or = 30% (P = 0.001). Higher LPL level was found in patients with
unfavorable cytogenetic aberrations (deletion in 17p13 or 11q22) than those with
favarable cytogenetics (deletion in 13q as the sole abnormality) (P = 0.002).
LPL level was not significantly correlated with sex, age, and ZAP-70 protein (P
> 0.05). The level of TK1 was higher in CLL patients than that in normal control
(P < 0.05). Patients with higher level of absolute lymphocyte count (ALC),
lactate dehydrogenase (LDH), unmutated IgVH genes and ZAP-70 had higher levels
of TK1 than those with lower level of ALC, LDH, mutated IgVH genes and ZAP-70 (P
= 0.018, P = 0.018, P = 0.030 and P = 0.038, respectively). TK1 level had no
correlationship with sex, age, Binet stages, CD38, and cytogenetic aberrations
(P > 0.05). CONCLUSIONS: LPL expression and serum TK1 levels significantly
correlate with other CLL prognostic factors and could be predictive markers for
IgVH mutation status. LPL and serum TK1 might be applied to the assessment of
prognosis in CLL patients.
PMID: 19563027


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